The interval from LMF to estrus was 7.2 ± 0.8 d, and the duration of estrus averaged 44.1 ± 1.0 h.
RESULTS Although a total of 4.0 × 109 sperm were used in each AI for all treatments, the actual number of motile sperm inseminated was 0.8 × 109 ± 0.2 for P, 1.2 × 109 ± 0.2 for M, and 1.7 × 109 ± 0.1 for G. Gilts assigned to treatment were excluded from analyses for abnormalities that included an EOI > 60 h (n = 12), ovarian cysts at estrus or at slaughter (n = 21), or uterine infection at slaughter (n = 2). Significant differences were identified at P ≤ 0.05 and trends at P > 0.05 and ≤ 0.10. The assumptions of ANOVA for normal distribution of data were evaluated and tested using PROC UNIVARIATE, and those for homogeneity of variance were evaluated and tested using Levene’s test.
Treatment did affect (P 0.10) and were removed from final models. Reproductive tracts were collected at approximately d 32 after AI. The insemination to ovulation interval (IOI) was determined using ultrasound every 12 h. For each treatment combination, a set of 3 boars was randomly selected within motility class for their allelic distinction with M sperm from a single boar represented across all treatments and sires used in both first and second inseminations. In replicates, mature gilts were synchronized and checked for estrus at 12-h intervals and assigned (n = 207) to receive 4.0 billion total sperm in each AI at 24 and 36 h after onset of estrus using the treatments: 1) P and M (P-M), 2) M and P (M-P), 3) G and M (G-M), and 4) M and G (M-G). To test the effect of variable FTS motility in a multiple-AI system, ejaculates from 38 boars were collected and frozen in 0.5-mL straws. Motility of FTS is related to in vitro and in vivo fertility, but this effect has not been determined in relation to the timing of ovulation. Knox2* *Department of Animal Sciences, University of Illinois at Urbana–Champaign, Urbana 61801ĪBSTRACT: Frozen-thawed boar sperm (FTS) has reduced motility and viability compared to cooled semen. Today, Minitube's product portfolio consists of all the necessary machinery, equipment and consumables to meet the individual needs of large, medium sized and small boar stud operations.Effect of using frozen-thawed boar sperm differing in post-thaw motility in the first and second inseminations on pregnancy establishment, litter size, and fetal paternity in relation to time of ovulation1 K. Another milestones in porcine AI technology development were the world’s first long-term extender for boar semen, Androhep®, in 1988 or the first fully automatic boar semen packaging (BSP) line worldwide only one year later. Simmet’s enthusiasm, one of the first boar studs in Germany was established soon after.Īs a pioneer in the field of artificial insemination, Minitube has provided expertise and high quality products to the boar stud and sow farm for five decades.Ĭare for an example? In 1972, with the Spirette®, Minitube developed the world’s first disposable AI catheter for pig insemination. Thanks to the foresight of the management at the lower Bavarian farmer's cooperative and to Dr. He wrote that he was “…immediately convinced that AI in pigs could offer farmers in Germany tremendous and immediate advantages.” In the same year, he and his wife Irmingard returned to Sweden to learn all they could about AI in swine. Ludwig Simmet went to Sweden in 1960 and was introduced to the AI techniques then being used in swine. However, Minitube did not play any less of a role in developing porcine reproduction technology.ĭr. The founding of Minitube in 1970 was closely related to automation of bovine semen processing with a novel straw technology.
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